FIG 2.

Drinking water biofilms on polyvinyl chloride surfaces. Biofilms were established on PVC BAR slides with uncolonized and colonized slides shown on the left and right, respectively (A). Biofilms were then inoculated with LpP1s1, fluorescently stained, and visualized using confocal microscopy. (B) From left to right, membrane-intact (green, SYTO 9), membrane-permeable (red, propidium iodide [PI]), and L. pneumophila (blue, Alexa Fluor 647) cells were observed by fluorescence microscopy, including an overlay of the signals. The top row shows stained, uninoculated biofilm controls (con), and the middle and bottom rows show LpP1s1-inoculated biofilms in the middle and edges of the BAR slide, respectively. Images are representative of four to six images collected from two independent experiments. Scale bars, 10 μm.