FIG 3.
sacB confers lethal sucrose sensitivity in GBS. (A) Stationary-phase WT GBS 10/84 or 10/84 transformed with plasmids used in this study was serially diluted 10-fold and plated on TS agar plates with appropriate antibiotic selection with or without supplemental 0.75 M sucrose. The first dilution shown is 10−1. (B) pMBsacB-based sucrose sensitivity functions in liquid culture and on solid medium, whereas pHY304 does not affect GBS survival in sucrose in either growth condition (***, P < 0.0001, analysis of variance [ANOVA]).