FIG 2.

Overexpression of adhE1 enabled C. cellulovorans to produce more n-butanol than overexpression of adhE2. (A) Effects of adhE1 and adhE2 introduction on butanol production. (B) Comparison of butyraldehyde dehydrogenase activities and butanol dehydrogenase activities between the ZQW02 and ZQW03 strains. (C and D) Fermentation profiles of ZQW01 and ZQW03, respectively, with AECC as the sole carbon source. The data in panels A and B are the means and standard deviations of three replicates (***, P ≤ 0.001; **, P ≤ 0.01; t test).