FIG 4.

MmsR activation of U737_12745 transcription in response to 3-OH-C₁₀-HSL or coculture with M. tundripaludum. Real-time qRT-PCR results showing relative expression of U737_12745, the first ORF in the neighboring gene cluster, for the wild-type LW13, ΔmmsR, and complemented ΔmmsR::mmsR strains in the presence versus the absence of 2 µM 3-OH-C₁₀-HSL (A) or the wild-type LW13 and ΔmmsR strains with versus without coculture with M. tundripaludum (B). Data are the mean ± standard deviation of three technical replicates and are representative of two independent experiments. *, significantly different (one-tailed homoscedastic t test, P value of <0.01); N.S., not significant.