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Experimental protocol scheme.
On the first day of the protocol, BrdU was injected (300 mg/kg, i.p) to all groups, and only the SD and MSD groups received SD. Melatonin was administered daily (10 mg/kg i.p.) at 8:00 a.m. After 96 hours (h) of SD, all groups are sacrificed for proper tissue processing. BrdU: 5-Bromo-2′-deoxyuridine; SD: sleep deprivation; MSD: melatonin; I.P. or i.p.: intraperitoneal.
