Skip to main content
. 2019 Oct;14(10):1726–1733. doi: 10.4103/1673-5374.257530

Figure 3.

Figure 3

Effect of VEGFA on the immunoreactivity and expression of claudin-5 in the hippocampus after SAH.

(A–D) Claudin-5-positive cells in the hippocampus in the sham (A), SAH (B), SAH + vehicle (C) and SAH + antagonist (D) groups. Positive endothelium was stained brown (red arrow). Endothelial damage is shown in the enlarged image (4× greater magnification, B and C). Area fraction was calculated in three regions of interest in each section, including CA1–3 and the dentate gyrus, using three coronal sections from each rat. (E) Western blot assay for claudin-5 expression. Claudin-5 is approximately 23 kDa and β-actin is approximately 42 kDa. (F) Protein expression levels of claudin-5 are expressed as a ratio of claudin-5 to β-actin levels for normalization. (G) Quantitation of claudin-5 immunoreactivity. Data are expressed as the mean ± SD (n = 5; one-way analysis of variance followed by the least significant difference test). **P < 0.01, vs. sham group; ##P < 0.01, vs. SAH + vehicle group. VEGFA: Vascular endothelial growth factor A; SAH: subarachnoid hemorrhage.