Table 2.

List of probes synthesized for in situ hybridization

Gene	Species	Target sequence	Primers
Tyrosine hydroxylase 1 (TH1)	Gallus gallus	NM 204805.2	Forward CATGTCTCCACGGTTCATTGReverse GACATGCCCCAGTAGCTCAT
Tyrosine hydroxylase 2 (TH2)	Gallus gallus	XM 001235000.3	Forward CTCTGCCGATGATTTTGATGReverse TCTATGCTGCTGGTGAATGG
Tryptophan hydroxylase 1 (TPH1)	Gallus gallus	NM 204956.1	Forward CAAGCTTTACCCAACTCATGCReverse TGGACCTCTCACTCTTCCATT
Tyrosine hydroxylase 1 (th1)	Xenopus tropicalis	ENSXETG00000014030	Forward CGGACCTGGATTTAGAGCACReverse CTCTTAGCCCGTCCAGAGAA
Tyrosine hydroxylase 2 (th2)	Xenopus tropicalis	ENSXETG00000025926	Forward AGGATCACCCTGGATTTGGTReverse GTTCCTCAGCTCGGCTTTTA
Tryptophan hydroxylase 1 (tph1)	Xenopus tropicalis	ENSXETG00000018984	Forward AACGACAATGTCTGCGAGAGReverse CAAGCCAATTTCTTGGGAGA