Table 2.
Application of AAV vectors at spinal cord and DRG level in pain researches
| Pain model or normal | Study sites | AAV serotype | Delivery method | Target gene | Reference | |
|---|---|---|---|---|---|---|
| Inflammatory pain | Induced by formalin | DRG | AAV6 and AAV8 | Intrathecal injection | Syntaxin 8 | Chen et al. (2014) |
| Neuropathic pain | Chronic constriction injury (CCI) | Spinal cord | AAV2 | Intraparenchymal injection | Brain‐derived neurotrophic factor | Eaton et al. (2002) |
| Intrathecal injection through lumbar puncture | Interleukin‐10 | Milligan et al. (2005) | ||||
| DRG | AAV6 | Direct injection | MiR‐7a | Sakai et al. (2013) | ||
| Spinal nerve ligation (SNL) | DRG | AAVrh20 and AAV1 | Intraneural (convection‐enhanced delivery, CED) | EGFP | Pleticha et al. (2014) | |
| AAV8 | Intrathecal injection through lumbar puncture | Prepro‐β‐endorphin | Storek et al. (2008) | |||
|
Spared nerve injury (SNI) |
DRG | AAV2 | Direct injection | Sodium channel Nav1.3 | Samad et al. (2013) | |
| Sciatic nerve injection | GTP cyclohydrolase I | Kim et al. (2009) | ||||
| AAV6 | Direct injection | Ca2+ channel‐binding domain 3 peptide | Fischer et al. (2014) | |||
| Bennett model and Gazelius model | Spinal cord | AAV2 | Intraneural injection | Agrin | Cui et al. (2010) | |
| Normal | DRG and spinal cord | AAV2 | Intrathecal injection | μ‐opioid receptor | Kao et al. (2010) | |
| AAV5 and AAV8 | Intrathecal injection through lumbar puncture | GFP | Vulchanova et al. (2010) | |||
| AAV8 | Intraperitoneal injection | GFP or LacZ | Zheng et al. (2010) | |||
| Intramuscular injection | GFP or LacZ | Zheng et al. (2010) | ||||
| AAV9 | Intrathecal injection | Superoxide dismutase‐1 (SOD1) | Hirai et al. (2012) | |||
| Spinal cord | AAV2 | Intraparenchymal injection | mu‐ opioid receptors S196A (MOR S196A) | Chen et al. (2010) | ||
| Subarachnoid injection | EGFP | Xu et al. (2003) | ||||
| AAV1, 2, and 5 | Intrathecal injection through lumbar puncture | EGFP | Storek et al. (2006) | |||
| DRG | AAV2 | Direct injection | mu opioid receptor | Xu et al. (2003) | ||
| EGFP | Xu et al. (2003) | |||||
| Sciatic nerve injection | EGFP | Xu et al. (2003) | ||||
| mu opioid receptors | Gu et al. (2005) | |||||
| Subcutaneous injection | EGFP | Xu et al. (2003) | ||||
| AAV6 | Intrathecal injection | EGFP | Towne et al. (2009) | |||
| Sciatic nerve injection | EGFP | Towne et al. (2009) | ||||
| Subcutaneous injection | EGFP | Towne et al. (2009) | ||||
| Intramuscular injection | EGFP | Towne et al. (2009) | ||||
| Tail vein injection | EGFP | Towne et al. (2009) | ||||
| AAV6 and AAV8 | Direct injection | EGFP | Yu et al. (2013) | |||
| AAV9 | Intraperitoneal injection | EGFP | Machida et al. (2013) | |||