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. 2018 Jul 13;126(4):263–271. doi: 10.1111/eos.12535

Figure 2.

Figure 2

Effect of L‐mimosine (L‐MIM) and hypoxia on core clock gene mRNA levels in three‐dimensional (3D) spheroid cultures of dental pulp‐derived cells (DPC). The DPC in 3D spheroid cultures were treated with L‐MIM or hypoxia for 24 h. mRNA levels of the genes circadian locomotor output cycles kaput (CLOCK) (A), aryl hydrocarbon receptor nuclear translocator‐like protein 1 (BMAL1) (B), cryptochrome circadian regulator 1 (CRY1) (C) and 2 (CRY2) (D) and period circadian regulator 1 (PER1) (E), 2 (PER2) (F) and 3 (PER3) (G) were quantified by quantitative PCR (qPCR). Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) was used as reference gene. Bars represent mean + SD, relative to the normoxic control (white bar). Experiments were conducted twice with DPC from three different donors (n = 6). *P < 0.05.