Figure 3.

Effect of L‐mimosine (L‐MIM), hypoxia and echinomycin on the levels of core clock proteins in two‐dimensional (2D) monolayer and three‐dimensional (3D) spheroid cultures of dental pulp‐derived cells (DPC). The DPC in 2D monolayer cultures were treated with L‐MIM or hypoxia (A) and with L‐MIM or hypoxia in combination with echinomycin or with echinomycin alone (C) for 24 h. Further DPC were cultured in 3D spheroid cultures and treated with L‐MIM or hypoxia (B). The amounts of circadian locomotor output cycles kaput (CLOCK), cryptochrome circadian regulator 1 and 2 (CRY1 and CRY2, respectively) and period circadian regulator 3 (PER3) proteins produced were detected by western blotting. Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) was used as reference protein. Experiments were conducted at least twice with DPC from two different donors (n = 4).