Figure 3.

Epicutaneous immunotherapy (EPIT) does not modify the proportions of Th1 and Th2 cells, but increases Foxp3⁺ Tregs, CD62L⁺, and CD62L⁻ Tregs obtained from spleen. Gating strategy (A and D) and analysis of the proportions of Th2 (B), Th1 (C) after a short in vitro stimulation with PMA‐ionomycin, and Foxp3⁺ Tregs (E), Foxp3⁺ CD62L⁺ Tregs (F), and Foxp3⁺ CD62L⁻ Tregs (G) ex vivo after EPIT. For CD4⁺, IL4⁺, and IFNγ⁺ gates, the y‐axis was arbitrarily defined with a fluorochrome different from those used in the gating strategy and without any specific antibody (PerCP‐Cy5.5 or APR‐Cy7). Results are expressed as mean ± SD in percentage of total CD4⁺ T cells. Differences between groups were analyzed by a Mann‐Whitney test. *P < .05, ***P < .001