Figure 6.

Demethylated CD62L⁺ Tregs confer protection against sensitization to a new allergen (peanut). (A) Experimental design for the evaluation of the bystander effect conferred by CD62L⁺ Tregs and CD62L⁻ Tregs after epicutaneous immunotherapy (EPIT). (B) and (C) serological response to sensitization to peanut expressed in μg/mL; (D) and (E) anaphylaxis was evaluated by measuring body temperature and the level of mMCP1 in plasma 30 min after intravenous injection of peanut solution in negative control, positive control, CD62L⁺ Treg EPIT, and CD62L⁻ Treg EPIT groups. Differences between groups were analyzed by a Kruskall‐Wallis test followed by Dunn's multiple comparison test. Individual data and medians are plotted *P < .05, **P < .01, ***P < .001