Figure 1.

Basal phosphorylation profiles in B cells, T cells and NK cells of pSS patients compared to healthy controls. Phosphorylation levels of NF‐κB, ERK1/2, P38, STAT4 Y693, STAT5 Y694, STAT1 Y701, STAT1 S727, STAT3 Y705 and STAT3 S727 were analysed by flow cytometry. (A) Phosphorylation levels (MdFI) of various epitopes and immune cells of pSS patient (black) and healthy donors (blue). Comparisons between pairs were performed using an Unpaired Mann–Whitney test. Median, 25 to 75 percentiles and maximum and minimum are shown. Differences were considered statistically significant when p ≤ 0.05, with significance indicated as *≤0.05, **≤0.01, ***≤ = 0.001 and ****≤ = 0.0001. (B) Loading plot for the PCA containing information about the variable for the corresponding PC, with the key to the right indicating cell type and phospho protein. Variables are given by vectors, with those contributing little to the PCA plotted around the centre as denoted by the grey axis, while variables that have high contributions are plotted further from the axes. After initial calculation of principal components the model was recalculated with only variables explaining greater than 50% of the variance retained. Groupings of samples by (C) disease status, (D) presence of SSA autoantibodies, (E) presence of extraglandular manifestations (EGM), and (F) medication status. Healthy donors = blue square, pSS patients = black circles, and pSS patients positive for SSA, EGM or medication (D, E and F, respectively) = red diamonds. The data represents 21 healthy controls and 24 patients pooled from 13 independent experiments.