Figure 3.

PCA representation of PIDOT results, showing the distinct blood leukocyte subsets in the reference data file and supervised analysis of blood samples from a healthy donor and three different PID patients. (A) Reference principal component analysis. PCA1 vs. PCA2 representation was generated from five healthy donors data files, analyzed with the EuroFlow PIDOT. (B) Different patient samples were analyzed against the healthy donor PCA reference. Blood samples from a healthy donor, a patient with mutated STAT3, a SCID patient with IL2 receptor gamma chain (IL2RG) deficiency, and a SCID patient with recombination activating gene 2 (RAG2) deficiency were stained and acquired under identical/comparable conditions. The STAT3 deficient patient shows the typical pattern of reduced naïve T-cells, virtually no IgH-class switched B-cells, and no eosinophils. The IL2RG deficient SCID patient has virtually no T-cells, particularly no naïve T-cells (<1 cell/μL; see Figure 1B), B-cells are present, but no memory B-cells or plasmablasts, and NK-cells are virtually absent. The RAG2 deficient SCID patient has no B-cells and no T-cells, while NK-cells are present in normal numbers. DNT, double negative T-cells, negative for CD4 and CD8, but positive for CD3.