Figure 9.

PCA representation of the biologically relevant subsets in a patient diagnosed with a WASp defect (5 years-old). The distribution of the biologically relevant PB lymphocyte subsets has been represented using multidimensional reference plots. The supervised analysis of the PID orientation tube using PC1 vs. PC2 reference plots (A) shows that low numbers of total and naïve CD8⁺ T-cells, and unswitched and switched memory B-cells, were detected in the patient sample after acquiring 1 × 10⁶ cells. Decreased counts of unswitched memory B-cells was confirmed in the analysis of the B-cell tubes (B). In addition, PCA1 vs. PCA2 representation of the T-cell tube (C) confirmed that naïve CD8⁺ T-cells were decreased.