Table 2.

Accuracy, matrix effects and recovery for the 35 metabolites of synthetic cannabinoids in urine. n = number of parallels. For concentrations of QC Low and QC High, see Table 1

	Accuracy (n = 10)		Matrix Effects (n = 10)				Recovery (n = 6)
Metabolite	QC Low	QC High	QC Low		QC High		QC Low		QC High
	%	%	%	CV (%)	%	CV (%)	%	CV (%)	%	CV (%)
AB‐PINACA pentanoic acid	102	119	–a	–a	123	119	98	13	105	33
AB‐CHMINACA M1A	95	117	–a	–a	57	59	106	4	105	12
RCS‐4 N‐(4‐hydroxypentyl)phenol	103	112	–a	–a	74	40	103	2	108	10
AB‐FUBINACA M2	84	111	–a	–a	228	63	105	8	87	15
5F PB‐22 3‐carboxyindole	92	100	101	24	88	14	106	6	103	22
RCS‐4 N‐pentanoic acid	95	115	88	33	108	27	106	7	99	11
PB‐22 N‐pentanoic acid	96	103	64	18	63	15	106	4	103	11
JWH‐250 N‐pentanoic acid	97	108	75	12	78	10	108	7	104	7
PB‐22 N‐(4‐hydroxypentyl)	96	108	62	15	72	12	101	5	98	8
JWH‐073 N‐butanoic acid	95	108	90	7	97	5	102	6	98	11
JWH‐203 N‐pentanoic acid	97	104	100	6	115	7	104	8	102	10
PB‐22 3‐carboxyindole	93	106	101	24	103	6	95	10	98	11
AB‐FUBINACA M3	119	135	115	19	156	16	108	7	102	8
AB‐CHMINACA 3‐carboxyindazole	92	112	106	24	133	11	105	5	106	5
JWH‐018 N‐pentanoic acid	107	100	94	33	117	7	98	9	95	11
AM‐2201 N‐(4‐hydroxypentyl)	98	98.9	146	15	175	12	99	6	97	9
JWH‐018 N‐(5‐hydroxypentyl)	107	108	83	19	84	16	84	9	84	11
JWH‐081 N‐pentanoic acid	106	102	114	18	123	9	91	12	97	9
AM‐2201 N‐(5‐hydroxyindole)	105	121	149	17	262	13	74	9	86	7
JWH‐122 N‐pentanoic acid	102	104	95	19	112	19	83	13	84	14
THJ‐2201 N‐pentanoic acid	101	113	195	22	220	28	96	9	96	9
BB‐22 3‐carboxyindole	79	109	–a	–a	114	10	84	9	93	8
JWH‐122 N‐(5‐hydroxypentyl)	102	110	177	28	176	31	70	8	79	6
AB‐PINACA COOH	91	113	144	27	143	23	100	9	100	7
UR‐144 N‐pentanoic acid	93	100	121	19	115	12	103	6	101	8
JWH‐210 N‐pentanoic acid	131	102	91	8	99	3	69	18	76	13
UR‐144 N‐(5‐hydroxypentyl)	98	102	118	10	118	7	84	5	88	7
UR‐144 N‐(4‐hydroxypentyl)	96	101	114	8	117	8	90	8	90	8
AKB48 N‐pentanoic acid	105	107	100	6	110	3	92	9	93	11
JWH‐210 N‐(5‐hydroxypentyl)	103	116	109	6	116	5	51	18	63	9
AB‐CHMINACA M2	97	101	95	21	104	4	94	14	94	10
5F‐AKB48 N‐(4‐hydroxypentyl)	95	104	112	6	118	5	88	9	88	8
AKB48 N‐(4‐hydroxypentyl)	93	111	102	4	109	4	76	7	79	8
AKB48 N‐(5‐hydroxypentyl)	95	110	111	5	115	8	80	9	82	9
JWH‐210 N‐(5‐hydroxyindole)	119	– b	89	10	93	5	11	56	17	25

^aMatrix effect was not estimated at low concentration.

^bQC High ended up outside of the linear range and data of accuracy are therefore left out.