Figure 5. Nrf2 inhibition by brusatol alleviates the antioxidant response induced by LBP (A) Effect of brusatol on the antioxidant response in LPS induced septic kidney. (B) Effect of brusatol on the Keap 1- Nrf2∕ARE signaling pathway related genes. (C) Western blot analysis of antioxidant responses and Keap 1- Nrf2∕ARE signaling pathway related proteins. (D) Quantitative analysis of the Western blot results. **, P<0.01, versus the DMSO group; ##, P<0.01, versus the LPS group; ΔΔ, P<0.01, versus the LBP group. DMSO control group (DMSO): intraperitoneal injection with DMSO. LPS model group (LPS): intraperitoneal injection with LPS (5 mg/kg); LBP-3 group: giving intragastric administration with 800 mg/kg LBP 1h after LPS injection; Brusatol group: intraperitoneal injection with LPS together with 2 mg/kg of brusatol, then LBP was administrated 1h after.