Figure 6. Polarized light image from the bone callus area. Type 1 collagen fibers are expressed in red-orange color and type 3 collagen fibers in yellowish-green. A- Control group, 28 days post-fracture. B- Dietary restriction group, 28 days post-fracture. Quantitative evaluation (%) of these images are reported in the graphs below, and are characterized by the decrease of the collagen type 1 (C) and collagen type 3 (D) fibers in the Res group. Asterisks indicate significant differences (p<0.05) (Picrosirus red, original magnification x100).