| Column | Chromolith SpeedROD RP–18e (50 mm × 4.6 mm i.d., Phenomenex Inc.) |
| Mobile phase | Methanol/ultrapure water/formic acid, 60:40:0.1, v/v/v |
| Elution | Isocratic |
| Flow rate | 0.5 mL/min |
| Injection volume | 5 μL |
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