Table 3. Effects of two different inhibitors of IDE on the kinetics of FBG degradation.

Kinetic parameters calculated from the data shown in Figure 4C, D. Note that P12–3A results in increased K_M^app values, consistent with reversible inhibition, while ML345 results in reduced v_max^app values, consistent with irreversible inhibition, in agreement with the established mechanisms of inhibition of the two inhibitors. Data are mean ± SD of 3 independent runs calculated from hyperbolic curves drawn through the raw S vs. v_o data (not from double reciprocal plots).

	DMSO	P12–3A	ML345
KMapp (nM)	331 ± 71	1540 ± 86	392 ± 73
vmaxapp (nM s−1)	3.37 ± 0.26	3.10 ± 0.15	0.827 ± 0.032