Figure 2.

A: Reaction scheme of the functionalization of alginate scaffolds (ALG) with tyramines using the carbodiimide chemistry (EDC/NHS). B: Wettability studies of modified scaffolds compared to standard cell culture‐treated polystyrene (PS) and native ALG. The coupling of 12.5 mg tyramine to 1 cm² ALG creates a surface wettability comparable to PS. C: Using zeta potential measurements, the surface charges of the scaffolds were analyzed. D: The elastic moduli describing the mechanical stabilities of the scaffolds were measured via compression tests. Alginate‐based hydrogels were found to be soft meeting the mechanical characteristics of human tissues. Differences of groups compared to ALG were considered significant by p < 0.01 (**) and p < 0.001 (***) (n.s.: not significant).