Figure 1.

miR‐365 expression was downregulated in liver CSCs. (A) The expression of miR‐365 in 100 pairs of HCC (T) and neighboring noncancerous tissues (N) was checked by real‐time PCR analysis. (B) The expression of miR‐365 in hepatoma spheroids was performed by real‐time PCR. (C) HCCLM3 and SMMC7721 cell‐derived spheroids were trypsinized and cultured in attachment conditions. miR‐365 expression in spheroids versus reattached cells was compared by real‐time PCR. (D) The expression of miR‐365 in CD133+ subpopulation of hepatoma cells was examined by real‐time PCR. (E) The expression of miR‐365 in EpCAM+ subpopulation of hepatoma cells was examined by real‐time PCR. (F) Real‐time PCR was performed to check the expression of miR‐365 in cisplatin‐resistant HCC xenograft