Figure 6.

JAK/STAT3 pathway was required for miR‐365 downregulation in liver CSCs. (A) HCC cells were treated with FH535 (40 nM), SIS3 (1 µM), S3I‐201 (100 µM) for 24 h and then subjected to real‐time PCR assay. (B) The expression of STAT3 in attached cells and hepatoma spheroids determined by Western bolt assay. (C) Hepatoma cells were transfected with si‐STAT3 and si‐NC for 24 h and then performed Western bolt assay. (D) HCC cells were transfected with si‐STAT3 and si‐NC for 24 hours. The miR‐365 expression was checked by RT‐PCR. (E) Hepatoma cells were infected with STAT3 overexpression plasmid or control plasmid for 24 h and then performed Western bolt assay. (F) HCC cells were infected with STAT3 overexpression plasmid or control plasmid for 24 h. The miR‐365 expression was checked by RT‐PCR