Figure 4.

Nuclear translocation of c-Fos and Nrf2 induced by OUFBW. The hPDLFs were stimulated with the medium, inactive OUFBW or OUFBW for 10 min. After the stimulation, the cells were incubated with the medium for 30 min or 6 h for evaluating the localization of c-Fos or Nrf2, respectively. Cells stimulated with 1 mM PMA for 30 min or 500 ng/mL TNF-α for 4 h were used as the positive controls. Cells were then fixed and immunostained with FITC-anti-c-Fos (a) or FITC-anti-Nrf2 (b). The nuclei were stained with DAPI (blue). Scale bars = 100 µm. (c and d) Percentage of the cells showing nuclear translocation of c-Fos (c) or Nrf2 (d). *p < 0.01 (Student’s t-test). Data are shown as means ± SD.