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. 2019 Jun 20;14(6):e0218091. doi: 10.1371/journal.pone.0218091

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© 2019 Joyce, Nour

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — A-Anti-TM219 antibody crosslinked to agarose beads and packed into a column was used to purify TM219 associated proteins from MCF7 cells treated (S) or untreated (C) with 1 μM IGFBP3. As a control we used normal mouse antibodies (N) conjugated to agarose beads. Eluted proteins were immunoprobed with anti-CaMKII antibody. Purified proteins from cells treated with IGFBP3 reacted specifically with anti-CaMKII antibody. These purified proteins were associated with also associated with calmodulin. B-Knockdown of CaMK II transcript using specific siRNAs diminished the phospho-beclin-1 signal in cells treated with IGFBP3 compared to MCF7 cells transfected with scrambled siRNAs or wild types.