Fig 1. Statistical analysis workflow outlining the steps taken to find predictors of HF test performance using metabolomic and lipidomic data.

Stepwise MRA was validated with bootstrap 95% confidence interval of the main regression estimates. Search of published threshold references confirmed the clinical importance of the models. Pathway enrichment analysis revealed the main metabolic pathways and associated diseases enriched using the set of metabolites predicting HF performance. A metabolic network was derived from the analysis confirming several metabolic dysfunctions related to HF described in the literature.