. 2019 Jun 20;14(6):e0218412. doi: 10.1371/journal.pone.0218412

Table 11. Mouse multiplex PCR assay.

Marker	Species	Chr Loc.	Forward Primer (5'-3')	Reverse Primer (5'-3')	Final [Primer] in 20μL (μM)
18–3	mouse	18	`[6FAM]-TCTTTCTCCTTTTGTGTCATGC`	`GTTTCTTGCTAAATAACTAAGCAAGTGAACAGA`	150
4–2	mouse	4	`[6FAM]-AAGCTTCTCTGGCCATTTGA`	`GTTCATAAACTTCAAGCAATGACA`	200
11–1	mouse	11	`[6FAM]-CCAGCCAGCCACTCTAGTTA`	`GTTTCTTACAGCTTCAGGACCATGGAA`	150
6–7	mouse	6	`[6FAM]-AGTCCACCCAGTGCATTCTC`	`GTTTCTTCATGTGGCTGGTATGCTGTT`	200
19–2	mouse	19	`[6FAM]-AGGCTAGCACTGTTCCTTGT`	`GTTTCTTACTCAGCACCTTCCATCCTG`	200
1–2	mouse	1	`[VIC]-TCTTTAAAAATCAAACAGGCAAA`	`GTTTCTTGGGGAGGTTGGGGTGTATAA`	225
7–1	mouse	7	`[VIC]-ACACCAGTTGGGGACTAGGA`	`GTTTCTTAACTGGTGCTGGGTCTGAAC`	200
1–1	mouse	1	`[VIC]-CCCTTCACTCCTTCATTCCA`	`GTTTCTTTGAGCCTAAGGACCTGGACA`	150
3–2	mouse	3	`[VIC]-TGAGCTACCATGTGGGTACA`	`GTTTCTTCACACACACACACAAAGATGGA`	200
8–1	mouse	8	`[VIC]-AGTAATATCCTGGTCCTGGCC`	`GTTTCTTGAGCTCACTATGTAGCTATTGGA`	200
2–1	mouse	2	`[NED]-ACAACTTCAAACTTTGCTGGAA`	`GTTTCTTTCCTGCTTAGCTTGTCATTTCC`	125
15–3	mouse	15	`[NED]-TCTGGGCGTGTCTGTCATAA`	`GTTTCTTAGGCTATCTAAACTTGACATGCT`	100
6–4	mouse	6	`[NED]-TTTGCAACAGCTCAGTTTCC`	`GTTTCTTAATCGCTGGCAGATCTTAGG`	200
11–2	mouse	11	`[NED]-AAGGCAGGGGAATTCACAGT`	`GTTTCTTTCTCACCATTGCAGTCCTGA`	200
17–2	mouse	17	`[PET]-CTCTTCTCCATCCGTCCATC`	`GTTTCTTATGGCTCTTGCCACAAATCT`	200
12–1	mouse	12	`[PET]-TTTCAAAATTGTCATTGAACACA`	`GTTTCTTTGGTCCTTCAGTATCATCCTTG`	200
5–5	mouse	5	`[PET]-CGTTTTACCTGGCTGACACA`	`GTTTCTTTGGTTTAAAACTCAATACCAAACAA`	250
X-1	mouse	X	`[PET]-AACAAAAATGTCCCTCAATGC`	`GTTTCTTAAGGTATATATCAAGATGGCATTATCA`	275
13–1	mouse	13	`[PET]-AACTCCCACTGCTTCTTGGT`	`GTTTCTTGCCAAAGTAAACCCTTCTCCC`	250
D8S1106	human	8	`[VIC]-TCTGCTGATTGTGCCCATTG`	`GTTTCTTATTGGCTGGTTAGTGACACA`	200
D4S2408	human	4	`[NED]-TCATTTCCATAGGGTAAGTGAAAA`	`GTTTCTTGCCATGGGGATAAAATCAGA`	250