Table 2. Lactonase activity of MoSMP30 and HuSMP30 in the presence of different metal cofactors.
| GTBL | MoSMP30 | HuSMP30 | ||||||
|---|---|---|---|---|---|---|---|---|
| Km (mM) |
Vmax (mM min-1) |
Kcat (min-1) | Kcat/ Km (mM-1 min-1) | Km (mM) |
Vmax (mM min-1) |
Kcat (min-1) | Kcat/ Km (mM-1 min-1) | |
| Ca2+ | 39.49±7.9 | 2.86±0.9 ×10−4 | 3.88 | 0.098 | 19.8 ±2.9 | 4.53 ± 2.7×10−4 | 6.12 | 0.31 |
| Co2+ | 19.17±7.88 | 8.57±3.5 ×10−4 | 11.65 | 0.60 | ND | ND | ND | ND |
| Mg2+ | ND | ND | ND | ND | ND | ND | ND | ND |
| Zn2+ | ND | ND | ND | ND | 9.68 ± 1.9 | 1.42±0.82×10−4 | 1.93 | 0.20 |
ND = Undetectable enzyme activity with 1mM metal concentration