Fig 1. Representative images of [³H] DAMGO binding in brain regions of interest at four bregma levels.

For ND (normal diet; no surgery), Sham (ad libitum high-fat diet; sham surgery), Sham FR (food restricted high-fat diet; sham surgery), and RYGB (ad libitum high-fat diet; RYGB surgery) rats. A quantitative binding scale of recorded standards is provided. Regions of interest are drawn on the atlas images adapted from the Paxinos and Watson rat brain atlas [36].