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. 2019 Jun 20;14(6):e0218569. doi: 10.1371/journal.pone.0218569

Fig 3. High resolution melt analysis for allele detection in the succinate dehydrogenase (Sdh) genes of Didymella tanaceti.

Fig 3

Left hand side of figure indicates primer binding regions and right-hand side shows normalised fluorescence for each of the known substitutions and wild-type in (A) SdhB codons 277 and 279; (B) SdhC codons 73 and 79; (C) SdhC codons 134 and 135 and (D) SdhD codons 112 and 122. On left hand side, black lines indicate the gene and arrows indicate the annealing position of primers (not to scale).