Table 4. Primers used for amplification of SdhB, SdhC and SdhD genes and High-Resolution Melt (HRM) assay.

Primer	Sequence 5’ - 3’	Use
Dt_SdhB_F	TACGTCAGGCTTTAGAAGGAAGGAG	SdhB gene
Dt_SdhB_R	GCCTAACTCAAAATACCAACTGC	SdhB gene
Dt_SdhC_F	GATACGC CCAAGACATCTACG	SdhC gene
Dt_SdhC_R	TACTCACTCCTCCAAATTCGTG	SdhC gene
Dt_SdhD_F	GCTCCACMGCTATCCTCG	SdhD gene
Dt_SdhD_R	TGGACACTTTAGCTTGTACTTTGCG	SdhD gene
SdhB _HRM_F	CTCAACAACAGCATGAGCTTG	SdhB HRM
SdhB _HRM_R	CAGCGCAGGGTTAAGTCC	SdhB HRM
SdhC_HRM_1F	CCATCTCACCATCTACCGC	SdhC HRM
SdhC_HRM_1R	GTAGAGGGAGCCGGAGAGC	SdhC HRM
SdhC_HRM_79_F	CGTCATTCAACCGTATCACCC	SdhC HRM
SdhC_HRM_2F	CTGAAGAGCTTCTTTGCGTTC	SdhC HRM
SdhC_HRM_135_F1	CCATGTTCTTCCACAGG	SdhC HRM
SdhC_HRM_135_F2	CCATGTTCTTCCACCGC	SdhC HRM
SdhC_HRM_2R	AGCAAATGCCTAACACCATTTGTTG	SdhC HRM
SdhC_HRM_QR_R	CCTAACACCATTGAAGCGTTG	SdhC HRM
SdhD_HRM_F	ATTCCCCTCACAGTTGCC	SdhD HRM
SdhD_HRM_R	TGATGCACGACTCGAAACC	SdhD HRM
SdhD_HRM_112_F	GCTGAACCCCGTCACCGAR	SdhD HRM