Fig 4. Assessment of apoptosis in hippocampal and cortical brain regions on postnatal day 2 in pups born of moms treated with ZIKV 107 or diluent control.
(A) There was no significant difference in apoptosis within the DG between ZIKV-infected and uninfected control pups (F1,10 = 0.053, P = 0.822). There was a significant increase in apoptosis per mm3 in the ZIKV-treated pups compared to the diluent control pups in the CA1 (B, F1,10 = 5.833, P = 0.036), CA3 (C, F1,10 = 10.784, P = 0.008), cortex (D, F1,10 = 11.813 P = 0.006), whole hippocampus (E, F1,10 = 21.102, P = 0.001) and the total apoptosis measured across all brain regions combined (F, F1,10 = 36.118, P < 0.001). (G) Representative images of cresyl violent stain in diluent pup and ZIKV pup brain, images taken in DG of the hippocampus. n = 6 animals per group. *: p < 0.05.