Fig 5. Investigation of microglial number and activation in hippocampal and cortical brain regions on postnatal day 2 in pups born of moms treated with ZIKV 107 or diluent control.
(A) There was a trend in the DG (F1,10 = 3.865, P = 0.078), indicating there are fewer microglia present in the ZIKV group compared to the diluent control group, though this effect was not significant. There was no significant effect of ZIKV infection on microglial numbers in (B) the CA1 of the hippocampus, (C) the CA3 of the hippocampus, (D) the hippocampus overall, (E) the cortex, (F) or in the analysis of all brain regions combined. Statistical analysis of microglial morphology revealed no significant difference in microglial morphology between treatment groups (G, Stout Microglia: F1,10 = 0.136, P = 0.720; H, Round Microglia: F1,10 = 0.067, P = 0.801). n = 6 animals per group. *: p < 0.05. (63X magnification, scale bar = 10μm).