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. 2019 Jun 10;17(6):e3000308. doi: 10.1371/journal.pbio.3000308

Fig 3. Adhesive properties of recombinant VAR2CSA WT, S429A/S433A, T934A, and T934D mutants.

Fig 3

VAR2CSA WT, mutants S429A/S433A (A), T934A (B), and T934D (C) were assayed by ELISA at different protein concentrations for in vitro binding to CSA- or decorin-coated ELISA plates. Increasing concentrations of recombinant proteins at serial dilutions of 0.156 to 10 μg/mL were added to wells previously coated with BSA, CSA, or decorin. Error bars correspond to SD between 3 independent experiments. Each experiment was performed in triplicate. Numerical values that underline the graphs are shown in S4 Data. BSA, bovine serum albumin; CSA, chondroitin sulfate A; Deco, decorin; ELISA, enzyme-linked immunosorbent assay; OD, optical density; VAR2CSA, variant surface antigen 2-CSA; WT, wild type.