Fig 2. Substrate binding of N2 VLPs via the 2SBS.

VLPs containing full length hN2 and aN2 were analysed for their ability to bind 3’SLNLN (A) or 6’SLNLN (B) in the absence or presence of OC using BLI as described in the legend to Fig 1. Similar amounts of N2 were applied based on the MUNANA assay as hN2 and aN2 proteins have identical enzymatic activities with respect to this substrate (Fig 1). Experiments were independently performed twice. Representative experiments are shown.