. 2019 Summer;13(3):25–34.

Table 3.

The primers and PCR conditions designed for the analysis of the MECP2 gene

Exon		Fragment		Primers	Product size(bp)	Tm(˚C)
Exon 1		Forward		CAAGCCTAGGCCTTCACTTGCC	610 bp	66 ˚C
Exon 1		Reverse		CATCCGCCAGCCGTGTCG	610 bp	66 ˚C
Exon 2		Forward		AGTGTGTTTATCTTCAAAATGT	376 bp	63 ˚C
Exon 2		Reverse		GTTATGTCTTTAGTCTTTGGG	376 bp	63 ˚C
Exon 3		Forward		CTTGCATGTGGTGGGGGTC	590 bp	63 ˚C
Exon 3		Reverse		AGTCATTTCAAGCACACCTGGTC	590 bp	63 ˚C
Exon 4	Exon 4a		Forward	GTTCAATAGTAACGTTTGTCAGAGC	841 bp	63 ˚C
	Exon 4a		Reverse	TGGTGGTGCTCCTTCTTGG	841 bp	63 ˚C
	Exon 4b		Forward	CTGGGCGGAAAAGCAAGGAGAG	545 bp	63 ˚C
	Exon 4b		Reverse	GTGATTTCAGTTAATCGGGAAGCTTTG	545 bp	63 ˚C