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. Author manuscript; available in PMC: 2020 Jun 21.
Published in final edited form as: Circ Res. 2019 Apr 9;125(1):14–25. doi: 10.1161/CIRCRESAHA.118.314223

Figure 2: miR-294 promotes cell cycle progression in cardiomyocytes.

Figure 2:

A) Increased expression of Ki67+/actinin+ cells was observed in NRVMs treated with miR-294–3p mimic compared to control mimic after 24hrs with quantification in (B) (n=5). Ki67 (green), α-actinin (red) and nuclei (blue). Scale bar = 40μm. C-D) NRVMs were labeled with EdU (G1/S transition) followed by miR-294 mimic or control treatment. EdU detection 24 hrs later showed increased % of EdU+/actinin+ cells in miR-294–3p group compared to control mimic. EdU (green), α-actinin (red) and nuclei (blue) (n=5). Scale bar = 40μm. E-F) Analysis of M-phase marker p-histone H3 showed increased %expression in miR-294 treated group compared to control 24hrs treatment. p-histone 3 (green), α-actinin (red) and nuclei (blue) (n=5). Scale bar = 40μm. Insets show higher magnification. G) Expression of aurora B in miR-294 treated NRVMs shows a late telophase NRVM stained for aurora B in the cytoplasmic body, (H) Significant increase in miR-294 group compared to control after 24hrs (n=5). Aurora B (green), α-actinin (red) and nuclei (blue). Scale bar = 20μm. I-J) Adult feline cardiomyocytes treated with miR-294 mimic show increased expression of p-histone H3 24hrs treatment. p-histone H3 (green), α-actinin (red) and nuclei (blue) (n=4). Scale bar = 40μm. K) miR-294 treatment of adult cardiomyocytes leads to significant % increase in mononucleated cells and a corresponding % decrease in binucleated myocytes (n=3). miR-Ctrl vs. miR-294 *p < 0.05, **p < 0.01, ***p < 0.001, data was assessed using unpaired student’s t test.