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. Author manuscript; available in PMC: 2019 Aug 28.
Published in final edited form as: Oncogene. 2019 Feb 28;38(25):4977–4989. doi: 10.1038/s41388-019-0768-8

Figure 3. Knockdown of c-Myc blocks enzalutamide/abiraterone upregulation of AR-FL/AR-V7.

Figure 3.

A & B, qRT-PCR (A) and Western blotting with a pan-AR or AR-V7 antibody (B) showing that c-Myc knockdown blocked enzalutamide (Enz) induction of AR-FL mRNA as well as AR-V7 mRNA and protein expression in VCaP cells. Cells were treated with 10 μM Enz at 24 h after shCtrl- or shMyc-lentivirus transduction. C, Western blot analysis showing loss of ability of abiraterone (Abi) to induce AR-V7 expression after c-Myc knockdown in LuCaP 35CR xenograft tumors. Right panel, quantitation of AR-FL and -V7 protein levels. *, P < 0.05.