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. Author manuscript; available in PMC: 2019 Aug 28.
Published in final edited form as: Oncogene. 2019 Feb 28;38(25):4977–4989. doi: 10.1038/s41388-019-0768-8

Figure 5. c-Myc positively regulates AR activity.

Figure 5.

A, qRT-PCR showing a downregulation of AR-FL and AR-V targets, PSA, UBE2C, CROT, and SOX9, but not the non-AR target PCP4 in shMyc-lentivirus-transduced cells compared to the control cells. *, P < 0.05 from the shCtrl group. B, Co-immunoprecipitation with a c-Myc antibody showing no direct association between c-Myc and AR-FL or c-Myc and AR-V7 in VCaP cells. Immunoblotting with a Max antibody was included as a positive control. C & D, Pearson’s correlation coefficient analysis showing a positive correlation between c-Myc level and AR activity and between c-Myc and AR activities in 159 mCRPC (C) and 1642 primary prostate cancer samples (D). The c-Myc and AR activity scores were computed as the sum of z-scores for the Schuhmacher c-Myc signature and the Nelson AR signature, respectively. E, GSEA showing enrichment of the hallmark androgen activity pathway in mCRPC samples that express a high level of c-Myc.