Skip to main content
. Author manuscript; available in PMC: 2019 Aug 28.
Published in final edited form as: Oncogene. 2019 Feb 28;38(25):4977–4989. doi: 10.1038/s41388-019-0768-8

Figure 6. c-Myc knockdown inhibits AR-gene transcription and destabilizes AR-FL and AR-V7 proteins.

Figure 6.

c-Myc knockdown was achieved by shMyc-lentivirus transduction. A, qRT-PCR showing a reduction of AR pre-mRNA after c-Myc knockdown. B, Luciferase assay showing decreased activities of AR promoters after c-Myc knockdown in 22Rv1 cells. Cells were transfected with the pGL4-ARpro8 or pGL4-ARpro1.7 construct in bulk and reseeded in triplicate 24 h post transfection for luciferase assay. C, mRNA stability assay showing no significant change in AR-FL or -V7 mRNA stability after c-Myc knockdown in 22Rv1 cells. Cells were treated with 10 μM actinomycin D for the indicated duration and collected for qRT-PCR. Right panels in A-C, Western blotting confirmation of c-Myc knockdown. D, Protein stability assay showing accelerated AR-FL or -V7 protein degradation after c-Myc knockdown in 22Rv1 cells. Cells were treated with 10 μg/ml cycloheximide (CHX) for the indicated duration and collected for Western blotting. Bottom panels, quantitation of AR-FL and -V7 protein levels. *, P < 0.05 from the shCtrl group.