Figure 2. Growth inhibition of selected agents against wild type and mcm2DENQ strains in MDT-replete (W303) and MDT-deleted (Δ3xMDT) strain backgrounds.

For assay trials, compounds were dissolved in DMSO (with the exception of hydroxyurea and XL413, which were dissolved directly in YPD, and cisplatin, which was dissolved in 0.9% saline) and diluted into YPD to their maximal solubility. Ten-point, two-fold concentration gradients were generated by serial dilution into YPD containing 1.9% DMSO, and transferred with a Biomek 2000 liquid handler to assay plates containing an optimized starting density of cells (inhibitor assay concentrations are shown in Table S1). Y-axis shows percent growth inhibition as defined in Materials and Methods using background subtracted Abs₆₀₀ values relative to cells that received vehicle only. All data points were at the highest concentration of compound tested (with the exception of 4-nitroquinoline 1-oxide, which is shown at its IC₉₀ (5 μM) and doxorubicin, which interfered with Abs₆₀₀ readings and was tested at the highest concentration that did not cause assay interference (150 μM). Each data point is the average ± S.D. of 1–7 independent experiments, performed in quadruplicate.