Table 1. Possible growth inhibition phenotypes and ability to detect them during HTS.

Table 1 provides a summary of possible growth inhibitory phenotypes; while single parameters can occasionally detect positives, only LDA captures all possible growth inhibitory phenotypes, which should permit discovery of a much wider range of agents and reduce the rate of false negatives. Please refer to the main text for more detail.

Growth Inhibition Phenotype	Expected Effect on Growth Curve Parameters			Synthetic Growth Defect captured by
	A (max. expansion)	λ (lag)	μ (slope)	A alone	λ alone	μ alone	LDA
Delayed growth with recovery over time	Same as control; time dependent	Increased	Similar or smaller	No	Yes	Yes / No	Yes
Delayed growth with sustained inhibition	Lower than control	Smaller or not measurable	Smaller	Yes	Yes / No	Yes	Yes
Acute toxicity with recovery over time	Same as control; time dependent	Increased	Similar or smaller	No	Yes	Yes /No	Yes
Acute toxicity with sustained kill	Lower than control	Smaller or not measurable	Smaller	Yes	Yes / No	Yes	Yes