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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Alcohol. 2018 Dec 21;80:5–16. doi: 10.1016/j.alcohol.2018.11.007

Figure 3. Fetal ethanol exposure decreased ZnT1 protein expression in the AMs from newborn pups but was restored by zinc treatment.

Figure 3.

Protein expression of zinc transporter, ZnT1, in alveolar macrophages isolated from pups was determined via immunostaining and quantified using fluorescent microscope and ImagePro Plus analysis. Bar heights represent mean RFU/field ± S.E.M. from at least 5 different litters per group (A). Representative fluorescent images are shown for each condition (B). CTRL = control group; CTRL + Zn = control AMs treated in vitro with zinc acetate; EtOH = ethanol group; and EtOH + Zn = ethanol AMs treated in vitro with zinc acetate. *** denotes p ≤ 0.05 when compared to the CTRL group and ** denotes p ≤ 0.05 EtOH + Zn vs. EtOH.