Fig. 2.

Colocalization of SVCT2 with organelle markers in breast cancer cell lines. (A) Immunofluorescence was performed in MCF-7, MDA-231 and MDA-468 cell lines. Cells were stained with anti-SVCT2 simultaneous with anti-COXIV, anti-LAMP2, anti-PDI, anti-RCAS1 or anti-GLUT1; and stained with Cy3 and FITC secondary antibodies. Samples were observed by confocal microscopy and 0.2-μm successive images were obtained along the z axis for further deconvolution analysis. (B) Pearson colocalization analysis in ImageJ using the JaCop plugin.