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. 2019 Jun 20;10:2701. doi: 10.1038/s41467-019-10427-2

Table 1.

The sequences of primers used for RT-qPCR analysis

Gene Organism Forward primer Reverse primer
GLUT1 Mus musculus 5′-CTTCACTGTGGTGTCGCTGT-3′ 5′-UUCUCCGAACGUGUCACGUTT-3′
HK2 Mus musculus 5′-TGATCGCCTGCTTATTCACGG-3′ 5′-AACCGCCTAGAAATCTCCAGA-3′
PFKL Mus musculus 5′-GGAGGCGAGAACATCAAGCC-3′ 5′-CGGCCTTCCCTCGTAGTGA-3′
PKM2 Mus musculus 5′-GCCGCCTGGACATTGACTC-3′ 5′-CCATGAGAGAAATTCAGCCGAG-3′
LDHA Mus musculus 5′-TGTCTCCAGCAAAGACTACTGT-3′ 5′-GACTGTACTTGACAATGTTGGGA-3′
PHGDH Mus musculus 5′-CGGCAGAATTGGAAGAGAGGT-3′ 5′-AGGAGTGGGGTATGGACAGTT-3′
PSPH Mus musculus 5′-CATCTCTGGTGGCTTTCGGA-3′ 5′-TTTCCTTTCCCACCCGACTC-3′
PSAT1 Mus musculus 5′-GGTGTGATTTTCGCTGGTGC-3′ 5′-AGGACTGATGGGCACTCTCT-3′
SHMT1 Mus musculus 5′-CAGGGCTCTGTCTGATGCAC-3′ 5′-CGTAACGCGCTCTTGTCAC-3′
SHMT2 Mus musculus 5′-ATGCCCTATAAGCTCAATCCCC-3′ 5′-TCTCATGCGTGCATAGTCAATG-3′
MTHFD1 Mus musculus 5′-GCGGAGAGGATGAGATCATAGA-3′ 5′-GTCACCCCGTCCACATCTT-3′
MTHFD2 Mus musculus 5′-AGTGCGAAATGAAGCCGTTG-3′ 5′-GACTGGCGGGATTGTCACC-3′
GAPDH Mus musculus 5′-AGGTCGGTGTGAACGGATTTG-3′ 5′-TGTAGACCATGTAGTTGAGGTCA-3′
GLUT1 Homo sapiens 5′-TCACTGTCGTGTCGCTGTTT-3′ 5′-GGCCACGATGCTCAGATAGG-3′
LDHA Homo sapiens 5′-GGCCTGTGCCATCAGTATCT-3′ 5′-GAAAAGGCTGCCATGTTGGA-3′
PHGDH Homo sapiens 5′-CACGACAGGCTTGCTGAATGA-3′ 5′-CTTCCGTAAACACGTCCAGTG-3′
PSPH Homo sapiens 5′-GCATAAGGGAGCTGGTAAGTCG-3′ 5′-ACCTGCATATTCACCGTTAAAGT-3′
HIF1A Homo sapiens 5′-ACCTTCATCGGAAACTCCAAAG-3′ 5′-CTGTTAGGCTGGGAAAAGTTAGG-3′
MYC Homo sapiens 5′-GGCTCCTGGCAAAAGGTCA-3′ 5′-CTGCGTAGTTGTGCTGATGT-3′
ATF4 Homo sapiens 5′-ATGACCGAAATGAGCTTCCTG-3′ 5′-GCTGGAGAACCCATGAGGT-3′
NRF2 Homo sapiens 5′-TTCCCGGTCACATCGAGAG-3′ 5′v-TCCTGTTGCATACCGTCTAAATC-3′
β-actin Homo sapiens 5′-GGGACCTGACTGACTACCTC-3′ 5′-ATCTTCATTGTGCTGGGTG-3′