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. 2019 Jun 20;9:8905. doi: 10.1038/s41598-019-45334-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of mice with inducible cartilage-specific IKKα knockout. Joint tissues retrieved from 12-week-old AcanCreER^T2/+; IKKα^f/f at 2 weeks after injection with vehicle (WT) or tamoxifen (cKO) were subjected to X-gal staining to assess tamoxifen-inducible cartilage-specific Cre recombinase activity. Representative images of the humerus, radius and ulna of the forelimbs obtained from WT and cKO mice are shown. (A) RTqPCR analyses in total RNA isolated from WT and cKO articular cartilage showed reduced IKKα mRNA in cKO samples (n = 2/ea) (B) and immunohistochemical analyses showed depletion of the IKKα protein in cKO articular cartilage (n = 4/ea). (C) Quantification of the positive immunostaining is shown on the left. Red = IKKα, Blue = DAPI. Scale bar = 10 μm **p = 0.0022 by one-sample t-test.