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. 2019 Jun 20;9:8836. doi: 10.1038/s41598-019-45237-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of UPR proteins in different glioblastoma cell lines non-treated and treated with Bortezomib using Orbitrap Fusion Lumos Tribrid Mass Spectrometer. Applying the optimized PRM assay, the expression level of the low abundancy UPR proteins at basal level and under treatment with 1 nM Bortezomib could be determined. The ratios between internal standard peptide and the endogenous peptide (light to heavy ratio) are presented. All the measurements were performed with three biological replicates.