Fig. 3.

NLRP3 immunoparalysis during sepsis is transitory. a Concentrations of C-reactive protein (CRP), procalcitonin (PCT), and IL-6 in plasma of septic patients at days 1, 3, and 5 during sepsis and at day 120 after sepsis recovery; dotted lines represent normal concentration of CRP and PCT. Levels of these markers in septic patients were compared with the abdominal surgery controls at 24 h after surgery. Control group and septic patients at day 1 correspond to patient data presented in Fig. 1a, b, and are shown here for comparison. b ELISA for IL-1β in PBMC supernatants (top) and percentage of monocytes with intracellular ASC specks (bottom) after NLRP3 inflammasome activation by LPS (1 μg/ml, 2 h) and ATP (3 mM, 30 min) treatment from control groups and NLRP3 non-immunocompromised (gray) or NLRP3 immunocompromised (blue) septic patients at day 1, 3, 5 during sepsis and at day 120 after sepsis recovery. Control groups and septic patients at day 1 correspond to patient data presented in Fig. 2a, b, and are shown here for comparison; each dot represents an individual patient; average ± standard error is represented in all panels; exact n number for each panel is presented in Source Data file; *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significant difference (p > 0.05); Kruskal–Wallis test for a, b