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. 2019 Jun 20;9:8832. doi: 10.1038/s41598-019-45216-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sequence similarity of IncHI2 plasmids from Irish farms with the pSTM6-275 plasmid. (A) The extent of the homology between the Irish IncHI2 plasmids with the pSTM6-275 was visualised with BRIG. Sequence assembly contigs from each sample were blasted against the pSTM6-275, which was used as reference. Each circle represents the plasmid from each of the 14 Irish isolates. The colour gradient indicates the degree of nucleotide identity. Genes in pSTM6-275 are represented by arrows (outer circle). Genes encoding antimicrobial or heavy metals resistance genes are annotated. A detailed list of gene presence/absence is present in Supplementary Table 4. (B) Phylogenetic relatedness of IncHI2 plasmids. Maximum-likelihood phylogeny of the thirteen IncHI2 plasmid sequences based on 162 core-genome SNPs identified using pSTM6-275 as reference. Due to the high proportion of missing data, plasmid from isolate 1495 C was excluded from the phylogenetic analysis.