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. 2019 Jun 20;9:8847. doi: 10.1038/s41598-019-45371-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental design and workflow for data analysis. The biological replicates, corresponding libraries, and sequence file labels are distinguished by colours and font type. Italics = samples run on Illumina GAII. Non-italics = samples run on Illumina HiSeq 2000; non-underlined samples were pooled into one sequencing lane (4), whereas underlined samples were pooled into another lane (5). Library labelled “Sample_12_TruSeq_12” is the result of combining total RNA from Hyd1 biological replicates 2 and 3, and thus considered a technical, rather than biological, replicate. This library was excluded from the differential expression analyses.